Why is concerned Proteome and Phosphoproteome, just focus on one gene and protein similar to the elephant, it is more concerned about the study of the entire system rather than a single protein and gene
probe capable of using mass spectrometric detection
chemical reporter (analogies sugar, Lipid azide and alkyne groups molecular wt looked like, and wt is the same behavior in the cell) - according to the chemical reaction - disadvantages: measuring the mass spectrometer
If made analogies with the amino acid, amino acid essential to the best choice, only added to the list by the in vitro amino acid
rather special Argine, a conditional essential amino acid
most commonly used is lysine
for most purposes like amino acids, tRNA unrecognized, need to re-identify tRNAenginering annalogies, but if the above acid is Change isotopes, e.g. c12-c13, n14 n15-, this is the case, the molecular structure and chemical properties substantially no difference, except that the mass
Probe - stable isotope labeled
corresponding to the cell medium and serum for a modified
SILAC medium
dialyzed serum
SILAC Amino Acids
after incorporation of isotopically labeled amnio acid cells, enzymatic put into the mass spectrometer, SILAC used for quantitative protein
SILAC other applications that protein interaction analysis of
cells infected with a virus after all changes of intracellular protein
quantification redox regulation during senescence (modified cys residues, etc.) redox regulation of protein analysis
taking antioxidant drugs do not contribute to longevity
Methods longevity:
caloric restriction / exercise more / NAD metformin melon ------ ROS will generate a large number of
the original point of view - an increase in oxidative aging body -
next to the cys arg is a basic group susceptible to oxidation; - oh readily basic group is easily oxidized by nucleophilic attack
next cys if one acidic group is not easily oxidized
This peptide CPT with titanium dioxide and iron enriched beads
Conclusion:
1. Low ROS mito peroxide, which golgi;
Why: that multiple ROS, reactive oxygen ligand protein, i.e. protein plurality antioxidant, oxidation degree is low;
2. aging, no increase in the degree of oxidation , but the site has changed a lot
to say: the same protein, different individuals in the same age when young and oxidized sites on the function of the protein is not old age is not the time to undertake different functions.
The impact of aging on metabolism
metabolites in the serum among the young and the aging time is very different - you can read the related article
mTORc1 pathway increased cell activity
Metabolism of tumor cells by regulating the immune
Metabolic pathways important areas: isotope tracing
Mass spectrometry - Chromatography
isotope tracing
can see which channel via which the activation suppressing
SILAC- have a certain impact on cell growth, cell culture such that the influence of the received smaller, lighter standard - the experimental group, rescaling -control
