1. The work flow detection site of PTM: Bottom-up Top-Down and
liquid chromatography mass spectrometry system, once a search library LC-MS- -protein ID
Question: trypsin digestion, why use tandem mass spectrometry search, the number of modifications to the test?
Before ionizing energy is too high, the protein broken into pieces
Therefore, until detection of peptide was applied to a 1900 +
at 445.12 445.62 molecule has two
Each peptide is constituted by a set of peak
analysis struture
Tandem Mass Spectrometry
The two spectra Release sequence
shears cut. . .
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different 4 mass, the mass spectrum obtained with the best quality tandem mass spectrometry, can be obtained per 40 mass
56 around 10,000, since cell-dependent time-dependent at one time instant is limited, the limited protein translation
. 7
8 -monocarboxylic cells genomics
9 microgram need to obtain a proteomic
