The Institute of Science and Technology and the Institute of Genetics and Development of the Chinese Academy of Sciences cooperate to create high-quality new wheat materials

Article，2023-12-10，Theor Appl Genet， [IF 5.4]

DOI：https://doi.org/10.1007/s00122-023-04504-w

First author:Qiu Yuliang, Han Zhiyang, Liu Ningtao

Corresponding author:Ye Xingguo, Han Fangpu

- background -

Wheat is one of the main food crops in my country, and quality improvement has always been an important breeding goal of wheat. Although my country's annual wheat output is 138 million tons, ranking first in the world, it still needs to import 800-900 tons of high-quality wheat every year for the processing of flour for high-end bread, pastries and other flour products. Since the genetic variation of glutenin in wheat is relatively narrow, it is necessary to introduce high-quality glutenin subunits from wheat wild relatives to improve wheat processing quality.

On December 10, 2023, Ye Xingguo’s team from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and Han Fangpu’s research group from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, collaborated and published an article titled “Effects” online in the established international journal Theoretical and Applied Genetics. of Aegilops longissima chromosome 1S^l on wheat bread-making quality in two "types of translocation lines" research paper, which used chromosome engineering technology to create the wheat-Tall goatgrass 1BS·1S^lL translocation line and 1S ^lS·1BL translocation line, further backcrossing the two translocation chromosomes into Ningchun No. 4 and other excellent wheat varieties, and breeding A new translocation line with excellent agronomic properties was developed. It was found that the bread quality of the new translocation line was significantly improved compared with the background parents. The molecular basis of the quality improvement was preliminarily analyzed.

- result -

In preliminary work, the research team compared the wheat-Tall goatgrass 1S^l (1B) substitution line CB-SLB with wheat varieties. Westoniade hybrid immature embryos were tissue cultured to construct a clonal variant population. At the same time, tall goat grass 1S¹L and 1S were developed through rough transcription sequencing technology. ^lL and other chromosome-specific molecular markers were further used to screen out 1S from the clonal variant population by using molecular markers and fluorescence in situ hybridization techniques. >^lL translocation lines >x2.3*< /span>1By18 and 1Bx17 both Normal expression, while the HMW-GS encoding genes carried by the wheat 1BL chromosome army16*^l1Sand^l1SThe HMW-GS encoding gene carried on the L chromosome^lL translocation lines were screened, and chromosome in situ hybridization analysis confirmed the translocation chromosomes According to the type and composition, the frequency of translocation occurrence was 0.12% (1/831) (Figure 1-a, c) and 0.088% (1/1140) (Figure 1-b, d), respectively. SDS-PAGE analysis results showed that 1S^l, two wheat-Tall goatgrass 1BS·1S₄ and TC₃S·1BL translocation system. In this study, molecular markers were used to select clones from the clonal variant populations TC^l

Figure 1 Chromosome in situ hybridization identification of wheat-Aegilops 1BS·1S^lL translocation line

Use 1BS·1S^lL and 1S^l The S·1BL translocation line was used as the male parent to cross with Ningchun 4, Ningchun 50 and Westonia respectively. The F₁ generation directly used the above three The varieties correspond to backcrossing. Use molecular markers to detect individual plants in BC₁ and subsequent backcross generations, and select individuals carrying translocated chromosomes. The strains were continuously backcrossed for 5 generations and selfed for more than 3 generations. In each selfing generation, molecular markers were also used to select target individual plants. The two translocated chromosomes were backcrossed into the genetic backgrounds of Ningchun 4, Ningchun 50 and Westonia, respectively, and wheat-Aegilops 1BS·1SL and 1S^lS·1BL. Bread quality analysis results show that the bread of the new translocation line (Figure 2, columns 2-6) is larger and more delicate than the recurrent parent (Figure 2, column 1), especially 1S^lS·1BL new translocation line (Figure 2, columns 4-6) has a more obvious effect on bread quality improvement. The results of 40K chip analysis showed that the wheat 1BL chromosome arm was missing in the new wheat-Aegilops 1BS·1S^lL new translocation line, 1S ^lS·1BL The wheat 1BS chromosome arm is missing in the new translocation line. The proportion of mSNPs in the new translocation line and the recurrent parent is 91.18-96.37%. The HMW-GSs content in the two types of new translocation lines is higher than that of the corresponding recurrent parents. The LMW-GSs and The gliadin content was significantly higher than that of the corresponding reincarnation parent. Transcriptome sequencing results showed that the expression levels of LMW-GSs and gliadin-encoding genes were up-regulated in the 1S^lS·1BL new translocation line. In summary, this study clarified the genetic effect of Aegilops elegans 1S^l chromosome on bread quality improvement, and provided new breeding materials for wheat quality improvement.

Figure 2 New translocation lines 1BS·1S with different genetic backgrounds^lL and 1S^{l< /span>}S·1BL Bread Processing Quality Analysis

Qiu Yuliang, a doctoral candidate who graduated from the Institute of Research and Development of the Chinese Academy of Agricultural Sciences, Han Zhiyang, a graduated master's student of Ningxia University, and Liu Ningtao, an associate researcher at the Heilongjiang Academy of Agricultural Sciences, are the co-first authors of this article. Ye Xingguo, a researcher at the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and a researcher at the Chinese Academy of Sciences Researcher Han Fangpu of the Institute of Genetics and Developmental Biology is the co-corresponding author of this article. This research was funded by the National Natural Foundation of China (31971945) and the Key R&D Program of Ningxia Science and Technology Department (2019BBF02020).

references

Qiu, Y., Han, Z., Liu, N. et al. Effects of Aegilops longissima chromosome 1Sl on wheat bread-making quality in two types of translocation lines. Theor Appl Genet 137, 2 (2024). https://doi.org/10.1007/s00122-023-04504-w

- Introduction to the corresponding author -

Institute of Crop Science, Chinese Academy of Agricultural Sciences

Ye Xingguo

researcher

Ye Xingguo, Ph.D., researcher. July 1980---August 1984: Majored in agronomy, Ningxia Agricultural College, and obtained a bachelor's degree; August 1988-July 1991: Majored in crop genetics and breeding, Graduate School of the Chinese Academy of Agricultural Sciences, and obtained a master's degree; August 1991 July - July 1994: Graduated from the Graduate School of the Chinese Academy of Agricultural Sciences, majoring in crop genetics and breeding, and received a doctorate.

Research directions: wheat cell engineering, genetic engineering and chromosome engineering breeding, including establishment of wheat transgenic system, development of chromosome markers of wheat related species, cloning of genes related to wheat regeneration and cultivation of new wheat strains, etc.

Academic part-time job: Editorial board member of BMC Genomics, BMC Plant Biology, Crop Magazine and Science and Technology Herald.

Honorary title: Young and middle-aged key talents of the Institute of Crop Science, Chinese Academy of Agricultural Sciences.

Award-winning achievements: 1 first-class Science and Technology Progress Award from Beijing, 1 first-class Science and Technology Progress Award from the Chinese Academy of Agricultural Sciences, and 1 second-class and third-class Science and Technology Progress Award from the Ningxia Hui Autonomous Region.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Korean Fangpu

researcher

Han Fangpu, Ph.D., researcher, doctoral supervisor. Obtained a PhD from the Institute of Genetics and Cells of Northeast Normal University; worked as a postdoctoral fellow at the Weizmann Institute in Israel from 1998 to 2001, engaged in research on wheat polyploid genome evolution; from 2001 to 2004, worked as a Visiting Fellow and Biologist at the Canadian Ministry of Agriculture, engaged in wheat resistance Research on scab molecular markers and germplasm innovation and wheat polyploid genome evolution; from 2004 to 2008, he worked on corn functional genome and plant artificial chromosome research at the University of Missouri-Columbia in the United States. Han Fangpu's research group is mainly engaged in research on wheat and corn functional genomes, wheat chromosome engineering breeding and plant artificial chromosomes.

write on the back

In order to encourage readers to communicate and quickly solve scientific research difficulties, we have established a "metagenomic" discussion group, which has been joined by 6,000+ scientific researchers at home and abroad. Please add the editor-in-chief meta-genomics on WeChat to bring you into the group, and be sure to note "name-unit-research direction-professional title/grade". Please indicate your identity if you have senior professional titles. There are also microbial PI groups at home and abroad for big bosses to cooperate and communicate with. To seek help with technical issues, first read"How to Ask Elegantly" to learn ideas for solving problems. Discussions in the group are still unresolved. Questions should not be chatted privately. Help peers. .

Click to read the original text and jump to the latest article list to read

The Institute of Science and Technology and the Institute of Genetics and Development of the Chinese Academy of Sciences cooperate to create high-quality new wheat materials

you may also like

write on the back

Guess you like