Moving Towards Third‐Generation Sequencing Technologies
迈向第三代测序技术
Within just the past few years, next‐generation sequencing (NGS) platforms have reduced the cost of DNA sequencing by several orders of magnitude. With these lower costs, DNA sequencing is increasingly powerful as a single‐data format onto which a broad range of biological processes can be projected for high‐throughput molecular profiling. Although significant challenges remain, translating these powerful technologies into useful laboratory tests has great potential. This chapter provides a comparative analysis of currently available NGS techniques, including those of Illumina, 454/Roche, Applied Biosystems, and Helicos BioSciences, and addresses emerging techniques, such as Pacific Biosciences single‐molecule sequencing and nanopore sequencing. Furthermore, very recent electronic sequencing from Ion Torrent is elaborated. The chapter discusses advantages and disadvantages of individual platforms, and those common to all techniques, including template preparation and genome enrichment.
就在过去的几年里，下一代测序(NGS)平台已经将DNA测序的成本降低了几个数量级。有了这些较低的成本，DNA测序作为一种单一的数据格式变得越来越强大，在这种数据格式上，可以预测出大范围的生物过程，从而进行高通量的分子分析。尽管仍存在重大挑战，但将这些强大的技术转化为有用的实验室测试具有巨大的潜力。本章对现有的NGS技术，包括Illumina、454/Roche、Applied Biosystems和Helicos BioSciences进行了比较分析，并介绍了新兴的技术，如Pacific BioSciences的单分子测序和nanopore测序。此外，还阐述了近年来离子激流的电子测序方法。本章讨论了单个平台的优点和缺点，以及所有技术的共同之处，包括模板制备和基因组富集。
参考文献
Ting Gao, Zhichao Xu, Xiaojun Song, Kai Huang, Ying Li, Jianhe Wei, Xunzhi Zhu, Hongwei Ren and Chao Sun, Hybrid Sequencing of Full-Length cDNA Transcripts of the Medicinal Plant Scutellaria baicalensis, International Journal of Molecular Sciences, 10.3390/ijms20184426, 20, 18, (4426), (2019).
