【课题】基于matlab GUI分水岭算法细胞分割计数【含Matlab源码 637期】

一、简介

分水岭算法是一种图像区域分割法,分割的过程中将图片转化为灰度图,然后我会将灰度值看作是海拔,然后向较低点注水,这种基于地形学的解释,我们着重考虑三种点:
在这里插入图片描述
极小值点,该点对应一个盆地的最低点,当我们在盆地里滴一滴水的时候,由于重力作用,水最终会汇聚到该点。注意:可能存在一个最小值面,该平面内的都是极小值点。
盆地的其它位置点,该位置滴的水滴会汇聚到局部最小点。
盆地的边缘点,是该盆地和其它盆地交接点,在该点滴一滴水,会等概率的流向任何一个盆地。
在这里插入图片描述
明白上述三种点之后,我们开始往盆地的极小值点注水,然后随着注水的深入,每一个极小值点慢慢的向外扩展,然后知道两个盆地的水汇合,汇合处就是我们需要的分水岭。

从下图可以直观理解一下,首先这三块区域都含有极小值点
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然后逐渐填充就能获得分水岭(即分界线)
在这里插入图片描述
得到分界线就能完成图像分割:
在这里插入图片描述

二、源代码

function varargout = CellCount(varargin)
% CELLCOUNT M-file for CellCount.fig
%      CELLCOUNT, by itself, creates a new CELLCOUNT or raises the existing
%      singleton*.
%
%      H = CELLCOUNT returns the handl e to a new CELLCOUNT or the handle to
%      the existing singleton*.
%
%      CELLCOUNT('CALLBACK',hObject,eventData,handles,...) calls the local
%      function named CALLBACK in CELLCOUNT.M with the given input arguments.
%
%      CELLCOUNT('Property','Value',...) creates a new CELLCOUNT or raises the
%      existing singleton*.  Starting from the left, property value pairs are
%      applied to the GUI before CellCount_OpeningFcn gets called.  An
%      unrecognized property name or invalid value makes property application
%      stop.  All inputs are passed to CellCount_OpeningFcn via varargin.
%
%      *See GUI Options on GUIDE's Tools menu.  Choose "GUI allows only one
%      instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES

% Edit the above text to modify the response to help CellCount

% Last Modified by GUIDE v2.5 01-May-2015 19:43:57

% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name',       mfilename, ...
                   'gui_Singleton',  gui_Singleton, ...
                   'gui_OpeningFcn', @CellCount_OpeningFcn, ...
                   'gui_OutputFcn',  @CellCount_OutputFcn, ...
                   'gui_LayoutFcn',  [] , ...
                   'gui_Callback',   []);
if nargin && ischar(varargin{
    
    1})
    gui_State.gui_Callback = str2func(varargin{
    
    1});
end

if nargout
    [varargout{
    
    1:nargout}] = gui_mainfcn(gui_State, varargin{
    
    :});
else
    gui_mainfcn(gui_State, varargin{
    
    :});
end
% End initialization code - DO NOT EDIT


% --- Executes just before CellCount is made visible.
function CellCount_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
% varargin   command line arguments to CellCount (see VARARGIN)

% Choose default command line output for CellCount
handles.output = hObject;

% Update handles structure
guidata(hObject, handles);

% UIWAIT makes CellCount wait for user response (see UIRESUME)
% uiwait(handles.figure1);


% --- Outputs from this function are returned to the command line.
function varargout = CellCount_OutputFcn(hObject, eventdata, handles) 
% varargout  cell array for returning output args (see VARARGOUT);
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Get default command line output from handles structure
varargout{
    
    1} = handles.output;



function edit1_Callback(hObject, eventdata, handles)
% hObject    handle to edit1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit1 as text
%        str2double(get(hObject,'String')) returns contents of edit1 as a double


% --- Executes during object creation, after setting all properties.
function edit1_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit3_Callback(hObject, eventdata, handles)
% hObject    handle to edit3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit3 as text
%        str2double(get(hObject,'String')) returns contents of edit3 as a double


% --- Executes during object creation, after setting all properties.
function edit3_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit4_Callback(hObject, eventdata, handles)
% hObject    handle to edit4 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit4 as text
%        str2double(get(hObject,'String')) returns contents of edit4 as a double


% --- Executes during object creation, after setting all properties.
function edit4_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit4 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit5_Callback(hObject, eventdata, handles)
% hObject    handle to edit5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit5 as text
%        str2double(get(hObject,'String')) returns contents of edit5 as a double


% --- Executes during object creation, after setting all properties.
function edit5_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit6_Callback(hObject, eventdata, handles)
% hObject    handle to edit6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit6 as text
%        str2double(get(hObject,'String')) returns contents of edit6 as a double


% --- Executes during object creation, after setting all properties.
function edit6_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end


% --- Executes on button press in pushbutton1.
function pushbutton1_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --- Executes on button press in pushbutton3.
function pushbutton3_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --- Executes on button press in pushbutton5.
function pushbutton5_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --- Executes on button press in pushbutton6.
function pushbutton6_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --- Executes on button press in pushbutton7.
function pushbutton7_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton7 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --------------------------------------------------------------------
function file_Callback(hObject, eventdata, handles)
% hObject    handle to file (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --------------------------------------------------------------------
function open_Callback(hObject, eventdata, handles)
% hObject    handle to open (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
global im;
[filename,pathname]=uigetfile({
    
    '*.jpg';'*bmp';'*.gif'},'选择图片');
%合成图片路径+文件名
str=[pathname filename];
%读取图片
im=imread(str);
%使用第一个axes
axes(handles.axes0);
imshow(im);


% --------------------------------------------------------------------
function save_Callback(hObject, eventdata, handles)
% hObject    handle to save (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)


% --- Executes on button press in pushbutton8.
function pushbutton8_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
close(gcf) %关闭当前figure句柄

% --- Executes on button press in pushbutton9.
function pushbutton9_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton9 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
global im
global I
global C
%%%%%%%%%%%%%%%%%%
I=im;
R=I(:,:,1);
G=I(:,:,2); 
B=I(:,:,3); 
K=rgb2hsv(I);           
H=K(:,:,1); 
S=K(:,:,2); 
V=K(:,:,3); 
[cenwhite,copywhite]=Kmeans(H,3);           
cenwhite;                       
A=copywhite; 
[m,n]=size(A);          
for i=1:m 
    for j=1:n 
        if A(i,j)==cenwhite(2) 
           A(i,j)=1; 
        else A(i,j)=0; 
        end 
    end 

三、运行结果

在这里插入图片描述

四、备注

完整代码或者代写添加QQ 1564658423

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转载自blog.csdn.net/TIQCmatlab/article/details/115267339