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Single-molecule sequencing technology has made significant breakthroughs
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| By the new DNA sequencing technology Oxford Nanopore Pacific Biosciences developed and can read a single DNA molecule sequences, and can be more clearly observed genomic organization structure and genetic content, however, single-molecule sequencing technology of this high error rate - Easy the nucleotide bases a DNA strand, T, G or C read error, the error rate of up to 15%. However, this situation will soon be changed. June 2012 issue of Nature Biotechnology magazine published entitled "Hybrid error correction and de novo assembly of single-molecule sequencing reads" article, researchers at Cold Spring Harbor Laboratory in New York would Pacific Biosciences single molecule sequencing technology and Illumina's traditional sequencing technology, and developed an open-source software PBcR for error correction in the single-molecule sequencing results show that the new method significantly improves the accuracy of the sequencing results. This method is also applicable to other single molecule sequencing techniques (such as Oxford Nanopore's sequencer), is expected to make single-molecule sequencing is widely used. In single-molecule sequencing technology represented a new generation sequencing technology (also known as "third generation" sequencing) can read the longer DNA strand, providing more information about the genetic patterns of gene structure and entire genomes, It can be used for sequencing large and complex genomes. The researchers developed the new method will help commercialize the company's Pacific Biosciences single molecule sequencer, and promote the development of single-molecule sequencing technology. However, a short time single-molecule sequencing technology will not replace traditional sequencing techniques, because the cost is not high when it is used for large and complex genome sequencing, but it is very useful to solve some specific problems. |
