将tif文件转换成mrc文件

https://www.nzwang-lab.net/2018/07/18/Tif2MRC/

---

title: “将tif文件转换成mrc文件”
subtitle: “tif 转换成 .mrc”
date: 2018-07-18
author: “ray”
tags:
- mrc2tif
- 技巧教程

---

首先将tiff堆转换成单页

---

path='/home/arine-lab/Reference/'; %原文件路径
savepath='/home/arine-lab/list_tiff/';   %新文件路径    

for i=1:100   %图片层数，可通过windows图片查看器知晓  
    I = imread([path,'NameOfTiff.tif'],i);   %读入文件的第i页
    imwrite(I,[savepath,num2str(i,'%04d'),'.tif']);   %保存单层图片，以其在原文件中的顺序命名
end

转换成mrc

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<http://bio3d.colorado.edu/imod/doc/man/tif2mrc.html>

    tif2mrc [options]  TIFF_input_files...  MRC_output_file

批量将tif 转换成mrc的脚本

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#!/bin/sh
#copy the exact files to a new dir ,and tif2mrc the files of the new dir

cd /home/list_tiff # home of tif pics 
mkdir ../tif_list # a dir for tif_lists

# 300 is the num of tif pics 
for((i=1;i<=300;i++)) 
do
    a=$((10000+$i))
    mkdir ../tif_list/${a:1}   
    for((j=i;j<i+25;j++))
    do
    b=$((10000+$j))
    cp ./${b:1}.tif ../tif_list/${a:1}/
    done

tif2mrc -d ../tif_list/${a:1}/*.tif  ../mrc_list/${a:1}.mrc
done

参考链接

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1. http://bio3d.colorado.edu/imod/doc/man/tif2mrc.html
2. https://hpc.nih.gov/apps/IMOD.html
3. http://bio3d.colorado.edu/imod/doc/mrc_format.txt