Glycated Albumin (GA) is an indicator that reflects the average blood sugar level in the past 2-3 weeks. The reaction cycle of glycosylated hemoglobin is shorter than that of the "gold standard" blood glucose test. Therefore, GA has advantages over A1c in confirming the therapeutic effect and adjusting the amount of clinical medication.
Glycated albumin is more able to reflect the recent blood glucose situation than glycosylated hemoglobin, can more accurately assess the recent treatment effect of diabetes, and is also helpful for the diagnosis and differential diagnosis of undiagnosed diabetes or stress hyperglycemia.
Method for determination of glycated albumin
⑴In the sample (serum or plasma), first inject glycosylated amino acid oxidase (KAOD: Ketoamineoxidase) to act to change endogenous glycosylated amino acids into glucketoaldehyde, amino acids and hydrogen peroxide and remove them.
⑵In the treatment solution, inject a protease specific to albumin, and produce from Glycated albumin (Glycated albumin).
⑶Secondly, inject glycosylated amino acid oxidase to produce glucketoaldehyde, amino acid and hydrogen peroxide from glycosylated amino acid.
⑷ Under the coexistence of TOOS and 4AAP, the generated hydrogen peroxide will quantitatively change into blue-purple pigment under the action of peroxide (POD: Peroxidase). The glycated amino acid produced from glycated albumin was quantified by measuring the absorbance of this blue-violet pigment.
The application of glycated albumin has gradually expanded to screening for diabetes, predicting the risk of chronic complications, assisting in the identification of stress hyperglycemia, and monitoring blood glucose during pregnancy, etc., and has become a new blood glucose indicator with clinical application value.
Abbexa Glycated Albumin and Human Glycated Albumin ELISA kits support the research of Glycated Albumin (GA) analysis.
Human Glycated Albumin (GA) ELISA Kit (abx252493)
Detection principle
The human glycosylated albumin (GA) ELISA kit is based on the sandwich enzyme-linked immunosorbent assay technology. The antibody is pre-coated on a 96-well plate. Add standards, test samples and biotin coupling reagent to the wells and incubate. Then add HRP-conjugated reagents and incubate the entire plate. Use washing buffer at each stage to remove unbound conjugates. The TMB substrate is used to quantify the HRP enzymatic reaction. After adding TMB substrate, only wells containing enough GA will produce a blue product, which then turns yellow after adding an acidic stop solution. The intensity of the yellow color is proportional to the amount of GA bound to the board. The optical density (OD) is measured spectrophotometrically at 450nm in the microplate reader, and the concentration of GA can be calculated from this.
Standard song of OD value of human glycated albumin (GA)
Human Glycated Albumin (GA) ELISA Kit Link:
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