Complement is a serum protein that exists in human and vertebrate serum and tissue fluids. It is not heat resistant, has enzyme activity after activation, and can mediate immune responses and inflammatory reactions. It can be activated by antigen-antibody complexes or microorganisms, causing pathogenic microorganisms to be lysed or swallowed. It can be activated through three independent and intersecting pathways, namely the classical pathway, the alternative pathway and the lectin pathway.
The complement system of higher mammals has three activation pathways: the classical pathway, the alternative pathway, and the lectin pathway. In the course of biological evolution, alternative pathways should appear as early as possible; secondly, lectin pathways should appear, and classical pathways should appear as late as possible.
Complement component C5 (usually abbreviated as C5) is the fifth component of complement, a part of the complement cascade, and plays an important role in inflammation and cell killing. It was found to be an inactive protein in the serum. The protein is composed of α and β polypeptide chains connected by disulfide bonds, but can be cleaved by one of two different C5 convertases (C4b2b3b or C3bBb3b). Two active peptides (C5a and C5b). Once lysed, the C5a fragment will act as an anaphylactoxin. It is derived from α polypeptides by the cleavage of C5 convertase, and can bind to C5a receptors on the surface of various white blood cells and trigger immune activation. C5b causes downstream complement proteins (C6-C9) to form membrane attack complexes (MAC) on the surface of pathogens. C5 mutations and defects are related to severe repeated infections.
Case study-Abbexa mouse complement C5 ELISA kit
Take abx153740 mouse complement C5 ELISA kit as an example:
The ELISA kit for mouse complement C5 is an ELISA kit used to quantitatively measure the concentration of mouse complement C5 in serum, plasma and other biological fluids in vitro. This assay has high sensitivity and excellent specificity for the detection of complement C5.
Detection principle of ELISA kit for mouse complement C5: This kit is based on sandwich enzyme-linked immunosorbent assay technology. The antibody is pre-coated on a 96-well plate. Add standards, test samples and biotin coupling reagent to the wells and incubate. Then add HRP-conjugated reagents and incubate the entire plate. Use washing buffer at each stage to remove unbound conjugates. The TMB substrate is used to quantify the HRP enzymatic reaction. After adding TMB substrate, only wells with enough C5 will produce a blue product, which then turns yellow after adding an acidic stop solution. The intensity of the yellow color is directly proportional to the C5 content on the board. The optical density (OD) is measured spectrophotometrically at 450nm in the microplate reader, and the concentration of C5 can be calculated from this.
The standard song of OD value of mouse complement C5-factory verification
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Complement C5 Analysis-Abbexa Mouse Complement C5 ELISA Kit
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