Biological safety cabinet is the main place treated cells, but also the main place of pollution cells, so the correct use of safety cabinet is the first step in the prevention of cell contamination. Then the security cabinet to note the small details, what does?
First of all, all the things into the cabinet before entering should be disinfected with 75% alcohol. Of particular note is our hand, as long as the security cabinet, and then into the spray must be 75% alcohol disinfectant before the security cabinet. Laboratory supplies try to lay in a suitable container after sterilization in newspaper well as * * head insertion head cartridge, other supplies may be placed in irregular aluminum boxes. * * When the head insertion head cartridge to gloves, sterilization may not be removed because the head may adhere * grease or other impurities.
Second, the security cabinet to be placed in an orderly things, this can be based on personal habits may be (if you are using a public safety cabinet, you may need to use the former temporary adjustment) to the Ming Zhou biological laboratories, for example, move and liquid head cartridge * (variety ready) placed on the right, the experiment required reagents to put the front right, front left discharge waste container, and the reagent and to ensure a certain safe distance, right corner of the dish is placed, is placed left corner other consumables (such as test tubes, etc.), try to ensure that the most common thing to put the right hand side (left-left and vice versa), once a common thing left discharge (prime ally opposite left), the right side of the operation area clean area, left a non-clean area ( The leftover waste container and other materials). Ming Zhou biological company laboratory trustworthy
Again, due to the process of using the security cabinet's hand and forearm are about to enter the cabinet, so the conditional shoes should be prepared at least one cell room dedicated Siamese clean clothes (after sterilization drying into a bag, in the cell room out, not out of the front room of non-replacement cells), no conditions comrades then at least for a pair of sleeves, each time after use to put safety cabinet UV disinfection.
Cell culture supplies and a variety of agents can not be separated, such as media, serum, dish cells / bottle or the like, so the second step is the prevention of contamination of cells to deal with and use of these reagents and consumables. If the laboratory conditions permit, the safest way to directly buy commercial reagents and consumables, such as Gibco, Hyclone, ScienCell, mingzhoubio reagents, Eppendorf, Corning supplies, etc., Ming Zhou biological reagents and consumables, quality did not problem. If you need to buy their own dry powder formulation reagents, reagent preparation in aseptic it is also particularly important. Further reagent preparation per recommended amount should not be excessive, such as the medium used up to 1-2 weeks is appropriate, then the other needle points may be considered a filtration filter, reduce the likelihood of bacterial contamination when loaded into the vial.
After the preparations are ready, the process of cell is we need to pay attention. When the cells were treated before the experiment should require various reagents, supplies ready in place, walking back and forth to minimize cell treatment process. Preferably prior to the cabinet using ultraviolet irradiation sterilization 30min, to ensure ventilation and ventilation 5min in place. During the experiment, all agents better not to close the bottle, open the bottle, if desired, then in addition to clean * head, not from anything else after the bottle just above, to prevent contamination of the reagent. The use of cell culture dish, it is best not to long open dish cover. Another way to hide the pipette is cell contamination, in particular the use of non-filter * comrades head, when drawing the reagent, it is easy to accidentally reagent sucked back into the pipette, if this happens, we must promptly with alcohol cotton ball wipe suck a reagent, in particular medium can easily become a breeding ground for bacterial growth. A contamination of the pipette can easily contaminate all cells of his comrades, and even entire laboratory cells, so the friendship is recommended conditional comrades must use the * head band filter in the treated cells. * Of course, even with the first band of the filter, should try to avoid reagent suck, because you want to completely remove the suction pipette pour reagents is very difficult.
As a cell culture incubator sites, also need to pay attention to his comrades. Due to the high temperature and humidity in the incubator, it is suitable for bacterial growth, so that if the conditions (laboratory incubator has two or more), preferably regularly with 75% alcohol wipe incubator, the incubator periodically replaced promptly the water tank.
So if the cells or unfortunately contaminated, then how should we do it? Ming Zhou Biological have a set of treatment measures, the laboratory cell contamination divided into early and late two kinds. Early cell contamination state is deteriorated, but still able to maintain growth, under a microscope (light microscopy) is not visible clearly visible small amount of a microorganism or a microorganism, which we proposed multi washed with PBS several times when changing the liquid, and then double antigen solution invasion 1-2min cells, removed by aspiration, normal growth medium was added. Early detection and timely as long as pollution, handled properly, he returned to the possibility is still very large. Late contamination is obvious change in cell morphology, cell has stopped growing, microorganisms can be seen clearly under the microscope. This time next boat biological https://www.mingzhoubio.com suggest that you clean out timely and relevant cells, in order to avoid contamination of other cells, revived an earlier batch of cells. Therefore, we will find that the new cell is received, should not rush to carry out experiments, but should first carefully cultured cells were frozen early 1-2 batches, and then carry out the experiment, once so experimental cell problem, you can stock cell ensure that experiments can continue.
These are clear biological boat to some of the ideas of pollution prevention and control cells, we have a problem you can go online to consult the company's official website: https://www.mingzhoubio.com If you feel good, give a praise, thank you!