It is a very effective method to make a bionic model to study the role of extracellular matrix in the differentiation of stem cells. Prepared arginine-glycine-aspartic acid short peptide (RGD) modified gold nanoparticles with adjustable surface ligand density to simulate the extracellular matrix microenvironment, and studied the effects of these nanoparticles on human bone marrow. The effect of osteogenic differentiation and adipogenic differentiation of mesenchymal stem cells. RGD-modified gold nanoparticles are phagocytosed by mesenchymal stem cells in a ligand density-dependent manner. RGD-modified gold nanoparticles, especially modified nanoparticles with high ligand density, have inhibitory effects on the alkaline phosphatase activity, calcium deposition and osteogenic gene expression of mesenchymal stem cells. When the RGD surface density is low, these nanoparticles have an inhibitory effect on oil droplet formation and fat gene expression, but when the RGD density is high, gold nanoparticles can promote oil droplet formation and fat gene expression. Biomimetic gold nanoparticles mainly regulate the osteogenic and fat differentiation of mesenchymal stem cells by affecting adhesion and cytoskeleton. This study clarifies the role of biomimetic nanoparticles in the process of stem cell differentiation, and will provide meaningful strategies for the design and manufacture of functional biomaterials for tissue engineering and biomedical applications.
The related customization list is as follows:
Multifunctional surface-modified metal nanoparticles,
carbon nanotubes/polyaniline/nano-gold composite materials,
coordination bond-modified functional nanoparticles/graphene oxide nano-hybrid materials,
bovine serum albumin-modified gold
nanoparticles, gold nanoparticles, functionalized reduction oxidation Graphene (RGO) and multi-walled carbon nanotubes (MWCNTs)
platinum modified dendritic gold nanocomposites
C18 bonded nano gold modified silica particles
graphene (GS)-chitosan (CS)-nano gold (Nano) -Au) composite
nano-gold (Au) coated clenbuterol - bovine serum albumin (CLB-BSA)
gold nanoparticles carboxymethyl chitosan
gold nanoparticles acetylcholinesterase
daunorubicin modified gold nanoparticles
gold nanoparticles Composite materials (ssDNA-AuNPs); Hyaluronic Acid-DNA hyaluronic acid modified deoxyribonucleic acid
Silica-coated gold nanoparticles
Amino and carboxyl functionalized gold nanoparticles
PEG-coated gold nanoparticles (PEG ends with different groups linked)
BSA Coated Gold Nanoparticles
Dextran Coated Gold Nanoparticles
Amino Functionalized Gold Nanoparticles
Carboxyl Functionalized Gold Nanoparticles
Polyethyleneimine Coated Gold Nanoparticles (PEI-nanogold)
Titanium Oxide Coated Gold Nanoparticles
Gold Nanoparticles in an Oxidizing Atmosphere Ion-coated silica shell core material
Red blood cell membrane coated gold nanoparticles
11-mercaptoundecanoic acid coated gold nanoparticles
Monodisperse PEG modified gold nanoparticles
Nano gold particles labeled exosomes
Glucose-coated Gold Nanoparticles (GNP)
Nano-gold particles label exosomes.
Silica-modified gold nanoparticles
D-alanyl-D-alanine (D-alanyl-D-alanine, DADA) modified gold Nanoparticles (Au_DADA) composite materials
Synthesis of Au-TDNNs composite materials on tetrahedral DNA modified gold nanoparticles
Arginine/amino acid modified gold nanoparticles Fullerol modified gold
nanoparticles
Peptide modified gold nanoparticles
FITC/carboxyl functionalized modification Gold nanoparticles
CY3/FITC/maleimide functionalized gold nanoparticles
CY3 and carboxyl functionalized gold nanoparticles
FITC and azide functionalized gold nanoparticles
Cy3 and NHS functionalized gold nanoparticles
FITC and methyl functionalized gold nanoparticles Particle
FITC and biotin functionalized gold nanoparticles
Cy3 and protein A functionalized gold nanoparticles
zzj 2021.3.17