RNA difficult group study of what? If the development of new technologies to address these difficulties , that you most want to solve the scientific problem?
RNA difficult research is to study technological backwardness
(1) from the flow of information, we need to direct determination of RNA sequences , but I have only indirectly measured by DNA sequencer.
(2) from the operating stream, we need to directly measuring the position of the modified nucleotide sequence , but reverse transcribed into cDNA after the lost original information modified nucleotides .
(3) We need to directly measuring the proportion of modified nucleotides , but there is necessary to measure each RNA sequence of the molecule and simultaneously determining the position and extent of the modifications nucleotides .
(4) We need to cells at the single cell level measured mRNA species and number , but now by cDNA measured as the actual measured quantity is very small.
(5) of each cell was measured on the single cell level RNA pool RNA distribution , but for the prior art for RNA quantity greatly.
(6) six technical areas of convergence (the fact is: these are the challenges of cutting-edge technology and key targets various fields of science)
In summary, the prior art require a lot of samples, the indirect sequencing, a single cell can not be achieved, the positioning of a single molecule, time, and quantity.
If the development of new technologies to address these difficulties , your most want to solve scientific problems based on RNA single straight measuring cell technology to solve the cancer type.