Scratch test
Use imageJ plug: Wound Healing Tool
download link
http://dev.mri.cnrs.fr/attachments/download/1992/MRI_Wound_Healing_Tool.ijm
installation method
Above file into the folder imageJ macros / toolsets, restart imagej.
Instructions
The rightmost point double arrow
Get this
Open a picture, left-click m can be measured directly, right-click to open the m parameter settings
The results are as follows
Click on the corresponding region number, a recording area on it (this is FIG. 2).
migration
Reference: http://www.sohu.com/a/240069404_650214
Quantitative process
Open a picture
shown in FIG, image-type-8-bit
image-adjust-threshold adjust the threshold value, the black cells of choice, there may be little cell behind can be screened out, click Apply.
Linked cell division
count
The adjustment to the parameters here
The results are as follows:
Angiogenesis
use tools
Angiogenesis Analyzer
http://image.bio.methods.free.fr/ImageJ/?Angiogenesis-Analyzer-for-ImageJ#outil_sommaire_0
下载地址
http://rsb.info.nih.gov/ij/macros/toolsets/Angiogenesis%20Analyzer.txt
installation method
Under the plug-in to download imagej folder / macros / toolset, the restart imagej.
Quantitative process
Open a picture, open Angiogenesis Analyzer
Direct quantitative
Change parameters
Quantitative CD31 blood vessels
Quantitative process
Open a picture
image-> Color-> Split Channels
FIG obtain the following three (red, green and blue channels)
This figure CD31 dyed red, the red channel can be retained.
Then image-> Adjust-> Threshold, hook Dark background, adjusted to the appropriate threshold point Apply
Analyze-> Set Measurements, the following options on the hook
Analyze-> Measure can be.
After the calculation of the results:
Reproduced in: https: //www.jianshu.com/p/f56ddab884ac