The golden age of three generations has arrived! In terms of amplicons, it has obvious advantages compared with the second generation:
1: The obtained sequence is longer, with more information and more accurate!
2. The identification is highly accurate, and the identification of species at the "species" level has been greatly improved.
3: PCR amplification has no GC preference, and the data is more accurate.
4: The whole area of V1-V9, more realistically restores the microbial community structure.
In 2023, Lingen Bio will comprehensively upgrade the PacBio Sequell platform, aiming to build a domestic first-class omics third-generation sequencing data production and data computing center, and provide high-quality third-generation sequencing data and scientific research services for scientific research users.
The following is a selection of the latest research progress of the three generations of full-length diversity. Welcome to leave a message below for exchange.
Front.Microbiol
原名： Heat stress weakens the skin barrier function of sturgeon by reducing mucus secretion and disrupting the mucosal microbiota
Time: 2022-8
Journal: Frontiers in Microbiology
Impact factor: 6.064
DOI： 10.3389/fmicb.2022.860079
Heat stress caused by global warming has damaged the living environment of aquatic animals, and skin tissue plays a vital role as a defense barrier to protect organisms. However, the response to heat stress in the skin of cold-water fish is poorly understood. In this paper, the effects of mild heat stress (24°C, MS) and high heat stress (28°C, HS) on the skin of Siberian sturgeon were studied using RNA-seq, histological observation, and full-length 16S diversity analysis.
in conclusion
1. In RNA-seq, 8819 differentially expressed genes (DEGs) between MS and C group and 12814 DEGs between HS and C group were obtained, among which there were 3903 DEGs between MS and C group and HS and C group, but only 1 652 DEGs were successfully annotated. Shared DEGs were significantly enriched in pathways related to mucin synthesis;
2. Histological observations showed that heat stress significantly reduced the number of skin and mucous membrane cells and induced epidermal damage;
3. The full-length 16s results showed that heat stress significantly destroyed the diversity and abundance of skin microbiota by increasing potential pathogens (Vibrio, Mesorhizobium, etc.) Spend;
4. The study reveals that heat stress has adverse effects on sturgeon skin, which is reflected in reduced mucus secretion and disordered mucosal microbiota, which may help to develop preventive strategies against global warming-induced heat stress.
Food Funct
Translation name: Menghuaside improves colitis in mice by improving intestinal barrier, inhibiting inflammatory response and regulating intestinal flora
Time: 2022-7
Journal: Food & Function
Impact factor: 6.317
DOI：10.1039/d2fo02128e
Menghuaside is a natural flavonoid compound, which exists in wild chrysanthemum, peppermint and other plants, and has various biological activities. The purpose of this article is to explore the protective effect of sucrose supplementation on dextran sodium sulfate (DSS)-induced colitis in C57BL/6J mice by using histological observations, physical and chemical experiments, qRT-PCR, western blotting and full-length 16S diversity. its underlying mechanism.
in conclusion
1, 25, and 50 mg/(kg·d) doses of mongoside could alleviate the histopathological damage caused by DSS, and improve the mucosal layer and intestinal barrier function.
2. 50 mg/(kg·d) mongoside reversed DSS-damaged intestinal flora, including Alistipes , Rikenella and Clostridia UCG-014 _norank. At the same time, it partially increased the relative abundance of short-chain fatty acid (SCFAs) producing bacteria, including Lactobacillus, Roseburia , Parabacteroides and Blautia , and increased the content of SCFAs.
3、蒙花苷可减轻 DSS 诱导的小鼠结肠炎，表明蒙花苷可能是减少炎症性肠病的一种有前景的营养策略。
INT J BIOL SCI
译名：通过特异性敲除 CIDEC 抑制磷脂酸的合成来改善肥胖和肝脏脂肪变性
时间：2022-9
期刊：International Journal of Biological Sciences
影响因子：10.75
DOI：10.7150/ijbs.74348
小肠是外源性脂质消化吸收的主要场所，它对脂质代谢稳态具有重要意义。在肠道以外的组织的脂质代谢中，细胞死亡诱导 DNA 效应因子 C(CIDEC) 具有活性。文章通过 Cre/LoxP 重组建立小肠特异性 CIDEC(SI-CIDEC-/-) 敲除 C57BL/6J 小鼠，通过组织学观察、理化试验、蛋白印记和全长16S多样性的方法探讨肠道 CIDEC 对脂质代谢的体内影响。
结论
1、与野生型 (WT) 小鼠相比，8周龄CIDEC小鼠喂食高脂饲料14周，体重降低15%，体脂量降低30%，甘油三酯 (TG) 降低79%，效果显著。
2、用16S rRNA 基因测序检测了小鼠新鲜粪便中微生物组的变化，发现α多样性不存在显著差异。两组的β多样性无显著差异。LEfSe 分析发现两组15种细菌存在显著差异，主要属于厚壁菌门、拟杆菌门和变形菌门。结果表明，饮食诱导的肥胖和肝脏脂肪变性的改善并不是由肠道微生物组的变化导致的。
3、机制研究表明，CIDEC通过与1-酰基甘油-3-磷酸-O-酰基转移酶相互作用促进 TG 积累，加速磷脂酸合成。
4、这项研究确定了一种新的相互作用蛋白和之前未报道的 CIDEC 机制。
J AUTOIMMUN
译名：ETS-1 通过上调 CIRBP 促进炎症性肠病 Th1 细胞介导的黏膜炎症
时间：2022-9
期刊：Journal of Autoimmunity
影响因子：14.511
DOI：10.1016/j.jaut.2022.102872
作为人类炎症性肠病 (IBD) 的易感基因， E26-1 癌基因同源物(ETS-1) 如何调节肠粘膜免疫应答尚不清楚。文章研究了 ETS-1 在 IBD 发病机制中的潜在作用。组织学观察、理化试验、免疫实验，qRT-PCR和全长16S多样性检测 IBD 患者 ETS-1 表达。
结论
1、健康对照组相比，活动性 IBD 患者CD4+T细胞中 ETS-1 表达显著增加，TNF-α可上调 ETS-1 表达，但抗TNF-αmAb治疗可显著抑制 ETS-1 表达。
2、Ets-1 Tg 小鼠更易发生 AOM/DSS 诱导的CAC，骨髓嵌合体进一步证明固有层免疫细胞而非肠上皮细胞有助于结肠炎的发生。
3、数据确定 ETS-1 在 IBD 患者中高表达，并通过 CIRBP 促进 Th1 驱动的粘膜炎症。CIRBP可作为治疗人类 IBD 的新治疗靶点。
参考文献
[1]Yang S,Xu W,Tan C, et al. Heat Stress Weakens the Skin Barrier Function in Sturgeon by Decreasing Mucus Secretion and Disrupting the Mucosal Microbiota. Front Microbiol. 2022;13:860079.
[2]Jin C,Liu J,Jin R, et al. Linarin ameliorates dextran sulfate sodium-induced colitis in C57BL/6J mice via the improvement of intestinal barrier, suppression of inflammatory responses and modulation of gut microbiota. Food Funct. 2022;13 (20):10574-10586.
[3]Huang L,Liao Q,Pan T, et al. Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid. Int J Biol Sci. 2022;18 (15):5740-5752.
[4]He Q,Gao H,Chang YL, et al. ETS-1 facilitates Th1 cell-mediated mucosal inflammation in inflammatory bowel diseases through upregulating CIRBP. J Autoimmun. 2022;132:102872.
凌恩生物全面升级的PacBio平台可满足16S全长研究需要，同时兼具时间短，无需扩增等优势，伴随着三代测序成本的下降，势必成为取代二代测序，提升研究品质的不二之选。欢迎来问~