In the process of flow cytometry experiments, the labeling effect of the fluorescent antibody on the single cell suspension directly affects the data quality of the experiment. Therefore, it is necessary to consider various factors that affect the quality and detection effect of flow cytometry antibodies, such as antibody specificity, fluorescein signal strength, fluorescein labeling method, isotype control, etc. This article describes how to choose the right flow cytometry antibody:
01
Flow cytometry antibody itself is also an antibody, so the selection of flow cytometry antibody must meet the most basic conditions for antibody selection: target protein specificity, reaction species, and application experiments.
02
The flow cytometry antibody fluorescent labeling methods include direct labeling and indirect labeling. In the flow experiment process, try to reduce the experimental procedures and processes to ensure the authenticity and accuracy of the experiment. Therefore, as far as the conditions allow, it is recommended to use directly labeled antibodies for experiments instead of indirect labeling.
Remaining content of the article<<<
Source: 51xxziyuan.com