background knowledge
Obesity is the excessive accumulation of fat caused by the imbalance between energy intake and consumption, which is caused by abnormal fat energy metabolism. It is a major risk factor for metabolic syndrome such as diabetes, hypertension, hyperlipidemia, fatty liver and cardiovascular disease. The different forms of adipose tissue distribution and function, the stored energy into white fat fat (mainly in mice eWAT abdominal fat) and energy consumption and heat production of brown fat (the BAT, located between the shoulder blades mice) and beige Fat (mainly iWAT, subcutaneous fat in mouse groin) [1-2] . Adipose tissue has been considered an endocrine organ due to its role in energy regulation, inflammation and immune response. In addition, it is also one of the sources of multifunctional cells with multidirectional differentiation potential , and this multifunctional cell exists in the vascular matrix (SVF, Stromal Vascular Fraction) part of adipose tissue [3].
SVF cells represent a heterogeneous group of cells, which surround the fat cells in adipose tissue. After cutting adipose tissue, digesting, and centrifuging, SVF can be obtained , which contains mesenchymal stem cells, endothelial precursor cells, macrophages, smooth muscle cells, pericytes, and abundant and highly plastic sources of adipose tissue Pre-adipocytes.
Preliminary preparation for primary extraction
1. Sterilized surgical instruments + small dishes containing 2ml DMEM basic medium
★ Surgical instruments
Including enough scissors and tweezers, do not mix instruments for different mice and different tissues. The instruments are sterilized in advance by high temperature and autoclave for later use.
★DMEM basic
Preheat at 37°C. Ensure the moist environment after the fat is separated .
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