Background knowledge to know
GEO=Gene Expression Omnibus
One gene corresponds to a GSE number. According to the number, find it in NCBI-GEO http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24673 (number according to need)
First of all, you must have a GES number, and then find
the usage of the expression matrix limma package.
The way to find differential genes is to change the R package to
understand the background knowledge of refseq, how the probe and the gene are perfectly matched (see the following blog),
learn the basics of the chip
a GSE There are three kinds of chip platform data how to deal with it
GEO database
Let’s take a look at the GEO homepage.
Each GES data
set
will have multiple
samples. Our starting point is a data set GSE, to figure out how many samples there are. One GSE will have multiple chip platform plantforms.
