new_dataframe = pd.DataFrame (columns = ['Longitude', 'Latitude'])
Assuming that there is already a list of longitude and a list of latitude, then
new_dataframe ['Longitude'] = lons
new_dataframe ['Latitude'] = lats
If the insert data is looped line by line, then
new_dataframe = new_dataframe.append ({'Longitude': 130, 'Latitude': 45}, ignore_index = True)