1, download the required software and database
nr database already, will not be discussed here
2, pfam database
wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pfam-A.hmm.gz
wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pfam-A.hmm.dat.gz
wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/active_site.dat.gz