Life Science Group:
For comparison of the respiratory chain eukaryotic gene is more genes encoding outer core, as related to the respiratory function of the mitochondria, nucleus located outside. Want to see whether the interaction of two genes, different species may encode ATP6 and ATP8 Construction of orthologous genes co phylogenetic tree; the heat can be plotted in FIG observed phenomenon block; correlation coefficient can be calculated; FIG box can draw mutual comparison between, for classes, a significant difference can be seen. The above operation can get a set value or FIG as determined whether the interaction, known data can be used to do the same operation, to obtain a standard value. Interaction between the two compared resulting composite.
The DIP (Protein Interaction Database) and PCN these existing database operator correlation coefficient. CoevDB is a known protein to its database to find protein interactions. PID is a protein-protein interaction of the information included in the database.
But there are false positive, false-positive reason is usually 1. small number of species, are not allowed to 2. find direct interaction but not functionally related proteins. Since relatively high false positive rate, it can be employed { experimental mass spectrometry and gene and protein} method for predicting a combination of mutual authentication.
It can be predicted using the principles of protein structure, protein-protein interaction prediction. There interaction databases using the template, the Y2H and functional domain interactions. The result is better is a direct membrane complex was built, but most can not achieve this effect. Other results are found both functional domain or a domain and interactions. Software can be used is RosettaDock Serve .
Small proteins predicted low potential, the amount of hours before the sample was considered non-coding an RNA , which can be found after the data after encoding the protein becomes large. Conserved region in which there is a hint functional importance. Less than check can check 30AA length of the domain, based on domain or a combination of the complementary functional duplication such that the genome becomes large, the proportion of the number of different amino acid changes during evolution of species, such as cysteine accounted for stabilizing increases ( its stabilizing compound having 1. a metal ion chelating 2. hydrophobic 3 disulfide bonds). Conservative protein compared to other proteins, conserved protein in accounting smaller; compared with the early origins of the new origin, the origin of new regulatory functions to increase the length of the variable length; the reason may be ancient proteins with common functions, and the new evolution of proteins with protein-specific , and continue to lengthen its structure.
Metagenomics and microbial diversity
Focused on microbial diversity of species abundance (ie, the number of species) and evenness (ie each number)
Metagenomic data available for uncultured species, i.e. not cultured in a laboratory environment. Therefore, metagenome be used in the pharmaceutical, environmental and disease areas. Technology used is based on the beginning 16sRNA , the back can be measured all the species of the Genome , which may eventually be sequenced according to the classification of microorganisms. But the difficulties encountered in metagenomics is because one-time measure to all genes in the environment, measured reads do not know what subspecies correspondence.