The advantages and disadvantages of various common methods of RNA interference are as follows:
(1) Chemical synthesis of dsRNA:
Advantages: Fast, usually you can get customized RNA oligo from the supplier within 6-8 days.
Disadvantages: high price, long customization cycle.
(2) In vitro transcription of dsRNA:
Advantages: The price is relatively low.
Disadvantages: difficult and time-consuming operation.
(3) PCR preparation of transcribed DNAs encoding shRNA:
Advantages: Economical and faster.
Disadvantages: This kind of dsDNA is difficult to introduce into cells.
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